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AIM:To study the morphology and ontogeny of dendritic cells of Peyer’s patches in rats at different development periods. METHODS:The morphometric and flow cytometric analyses were performed to detect all the parameters of villous-crypts axis and the number of OX62+DC, OX62+CD4+SIRP+DC,and OX62+CD4-SIRP-DC in the small intestine in different groups of rats.The relationship between the parameters of villous-axis and the number of DC and DC subtype were analyzed. RESULTS:All morphometric parameters changed significantly with the development of pups in the different age groups(F=10.751,12.374,16.527, 5.291,3.486;P=0.000,0.000,0.000,0.001,0.015). Villous height levels were unstable and increased from 115.24μm to 140.43μm as early as 3 wk postpartum. Villous area increased significantly between 5 and 7 wk postpartum,peeked up to 13 817.60μm2 at 7 wk postpartum.Villous height and crypt depth ratios were relatively stable and increased significantly from 2.80±1.01 to 4.54±1.56,9-11 wk postpartum.The expression of OX62+DC increased from 33.30%± 5.80%to 80%±17.30%,3-11 wk postpartum(F=5.536,P=0.0013).OX62+CD4+SIRP+DC subset levels detected in single-cell suspensions of rat total Peyer’s patch dendritic cells(PP-DCs)increased significantly from 30.73%±5.16%to 35.50%±4.08%,5-7 wk postpartum and from 34.20%±1.35%to 43.60%± 2.07%9-11 wk postpartum(F=7.216,P=0.005). CONCLUSION:This study confirms the agerelated changes in villous-crypt axis differentiation in the small intestine.Simultaneously,there are also development and maturation in rat PP-DCs phenotypic expression.Furthermore,the morphological changes of intestinal mucosa and the development of immune cells(especially DC)peaked at 9-11 wk postpartum, indicating that the intestinal mucosae reached a relatively mature state at 11 wk postpartum.
AIM: To study the morphology and onto geny of dendritic cells of Peyer’s patches in rats at different development periods. METHODS: The morphometric and flow cytometric analyzes were performed to detect all the parameters of villous-crypts axis and the number of OX62 + DC, OX62 + CD4 + SIRP + DC, and OX62 + CD4-SIRP-DC in the small intestine in different groups of rats. The relationship between the parameters of villous-axis and the number of DC and DC subtype was analyzed. RESULTS: All morphometric parameters changed significantly with the development of pups in the different age groups (F = 10.751, 12.374, 16.527, 5.291, 3.486; P = 0.000,0.000,0.000,0.001,0.015). Villous height levels were unstable and increased from 115.24 μm to 140.43 μm as early as 3 wk postpartum. Villous area increased significantly between 5 and 7 wk postpartum, peeked up to 13 817.60 μm2 at 7 wk postpartum. Villous height and crypt depth ratios were relatively stable and increased significantly from 2.80 ± 1.01 to 4.54 ± 1.56 , 9-11 wk pos The percentage of OX62 + DC increased from 33.30% ± 5.80% to 80% ± 17.30%, 3-11 wk postpartum (F = 5.536, P = 0.0013) cell suspensions of rat total Peyer’s patch dendritic cells (PP-DCs) increased significantly from 30.73% ± 5.16% to 35.50% ± 4.08%, 5-7 wk postpartum and from 34.20% ± 1.35% to 43.60% ± 2.07% 9-11 CONCLUSION: This study confirms the agerelated changes in villous-crypt axis differentiation in the small intestine. Simultaneously, there are also development and maturation in rat PP-DCs phenotypic expression. Futurertherm, the morphological changes of intestinal mucosa and the development of immune cells (especially DC) peaked at 9-11 wk postpartum, indicating that the intestinal mucosae reached a relatively mature state at 11 wk postpartum.