论文部分内容阅读
[目的]利用近红外漫反射光谱技术建立快速简捷鉴别小麦抗旱性的评价方法。[方法]选择36个不同抗旱类型的小麦品种为试验材料，依据 GB/T 21127-2007小麦抗旱性鉴定评价技术体系规范要求，以抗旱指数为指标，在2007~2009年2个生长年度，对试验材料进行旱棚抗旱性分类分级鉴定研究，并利用 FOSS NIRSystems5000型近红外全光谱谷物品质分析仪，对旱棚收获后的小麦籽粒样品进行实验室光谱采集、建库和验证分析，同时进行定性分析和全光谱相关性研究。[结果]试验得到的拟合方程参数中的决定系数（ RSQ）=0.6975、交互检验决定系数（1-VR）=0.6002，方程拟合度高，品种间近红外漫反射光谱差异显著，且与抗旱指数具有显著或极显著的相关性。[结论]利用近红外漫反射光谱技术测定评价小麦的抗旱性具有简单方便、准确快捷、全籽粒无破损等优点，是一项检测小麦抗旱性切实可行的简捷技术，对全面建立小麦抗旱性评价指标技术体系和育种材料早代鉴别具有重要的实用价值。“,”[Objective] The aim was to build an evaluation method rapidly identifying wheat drought tolerance with near infrared diffuse reflectance spectroscopy. [Method] In the research, 36 wheat varieties in 2007-2009 were chosen and drought-tolerance degrees of wheat were graded and identified according to Winter-wheat Drought Tol-erance Evaluation Technical Standards (GB/T 21127-2007), and harvest wheat grains underwent spectrum col ection, with a ful-spectrum analyzer, to establish a database. [Result] Based on qualitative analysis and ful-spectrum correlation research, the coef-ficient of determination (RSQ) and cross-validation coefficient of determination (1-VR) were concluded at 0.697 5 and 0.600 2, showing near-infrared diffuse reflectance spectroscopy is of significant differences among wheat varieties and of significant or extremely significant correlation with drought-tolerance indices. [Conclusion] The re-search indicates that to evaluate drought-tolerance of wheat with near-infrared diffuse reflectance spectroscopy is a rapid and feasible way, which is simple, convenient without damages on grains, and of practical values for construction wheat drought-tol-erance evaluation index system and identification of breeding materials.