论文部分内容阅读
目的建立两种简便的白色念珠菌ＤＮＡ提取方法，即纯ＤＮＡ提取法和快速ＤＮＡ提取法。方法以原生质体提取法为基础进行改良，两种方法均用Ｌｙｔｉｃａｓｅ破壁。结果与结论纯ＮＤＡ提取法提取ＤＮＡ耗时６～７ｈ，产量较高，每１０~（１０）个菌细胞可提取４μｇＤＮＡ，且纯度高，ＯＤ_（２６０）ＯＤ_（２８０）＞１．７，对核酸限制性内切酶敏感，适用于各种分子生物学方面的研究。快速ＤＮＡ提取法全过程仅需３～４ｈ，每１０~（１０）个菌细胞提取１μｇＤＮＡ，ＯＤ_（２６０）：ＯＤ_（２８０）＝１．４，不能进行核酸限制性内切酶酶切，但可用于作ＰＣＲ扩增。
Objective To establish two simple DNA extraction methods of Candida albicans, namely pure DNA extraction and rapid DNA extraction. The method was modified on the basis of protoplast extraction, both of which were broken with Lyticase. RESULTS AND CONCLUSION Pure DNA extraction (NDA) method took 6 ~ 7 hours to extract DNA, yielding 4μg DNA per 10 ~ 10 cells with high purity and OD 260 (280)> 1.7 Nucleic acid restriction endonuclease sensitive, suitable for a variety of molecular biology research. In the whole process of rapid DNA extraction, it takes only 3 ~ 4h to extract 1μg DNA per 10 ~ 10 bacterial cells. OD_ (260) OD_ (280) = 1.4 can not be digested with restriction endonuclease Can be used for PCR amplification.