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采用传统的平板计数法和PCR凝胶电泳技术,在大田栽培条件下,以转BADH基因苜蓿和非转基因苜蓿为材料,于2009年和2010年连续2年测定苜蓿根际土壤细菌、放线菌和真菌数量的变化,并对外源基因是否转移到土壤微生物中的可能性进行检测分析。结果表明,在不同年份和不同月份转基因植株与非转基因植株根部土壤3大类微生物(细菌、放线菌和真菌)数量变化趋势一致;二者根部土壤3大类微生物数量无显著差异,2年间转基因苜蓿的土壤3大类微生物数量无显著差异;并利用BADH基因特异引物对土样的总DNA,以及菌株DNA进行PCR扩增,均未检测到外源基因扩增产物。初步研究结果表明,转基因苜蓿对土壤微生物系统尚没有明显的影响。
The traditional method of plate count and PCR gel electrophoresis was used to measure the rhizosphere soil bacteria and actinomycetes in alfalfa field in 2009 and 2010 with the transgenic BADH gene alfalfa and non-transgenic alfalfa under field cultivation conditions. And the change of the number of fungi, and detect whether the exogenous gene is transferred to the soil microorganism. The results showed that there was a consistent trend in the quantity of three kinds of microorganisms (bacteria, actinomycetes and fungi) in the roots and roots of transgenic and non-transgenic plants in different years and months. There was no significant difference in the quantity of three kinds of microorganisms in the soil between the two years There was no significant difference in the number of microorganisms in the soil from the transgenic alfalfa. The total DNA of the soil sample and the DNA of the strain were amplified by PCR using BADH gene-specific primers. Preliminary results show that transgenic alfalfa has no obvious effect on soil microbial system.