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[目的]观察亚砷酸钠(sodium arsenite,NaAsO_2)对Chang肝细胞株血红素单加氧酶-1(heme oxygenase-1,HO-1)mRNA和蛋白表达的诱导作用。[方法]以5μmol/L和10μmol/L的NaAsO_2作用Chang肝细胞株2、6、12h和24h,分别用逆转录-聚合酶链反应(RT-PCR)和WesternBlot法检测细胞内HO-1的mRNA和蛋白表达情况。[结果]5μmol/L和10μmol/L NaAsO_2暴露2h开始出现HO-1 mRNA的诱导表达;6h的表达水平明显高于对照组和2h暴露组(P<0.01)。其中,10μmol/LNaAsO_2暴露12h和24h的mRNA表达均明显高于该浓度的6h暴露组(P<0.01);但24h的HO-1 mRNA表达水平与12h组相比没有明显升高。NaAsO_2暴露诱导的HO-1蛋白表达则从6h开始出现,12h组明显高于6h组,24h组明显高于12h组(均P<0.01);5μmol/L和10μmol/L NaAsO_2分别暴露6、12h和24h的HO-1蛋白表达量分别是对照组的2.80、9.34、18.15和3.97、12.92、23.29倍;此外,10μmol/L NaAsO_2暴露12h和24h的HO-1mRNA和蛋白表达均明显高于对应时间的5μmol/L组(P<0.01)。[结论]NaAsO_2暴露能够有效和持续性诱导Chang肝细胞株HO-1的mRNA和蛋白表达,是无机砷暴露的一种细胞保护性适应性反应。
[Objective] To observe the induction effect of sodium arsenite (NaAsO_2) on heme oxygenase-1 (HO-1) mRNA and protein expression in Chang hepatocyte cell line. [Method] Chang hepatocytes were treated with 5μmol / L and 10μmol / L NaAsO 2 for 2 hours, 6 hours, 12 hours and 24 hours respectively. RT-PCR and Western Blot were used to detect the expression of HO-1 mRNA and protein expression. [Result] The expression of HO-1 mRNA began to appear after exposed to 5μmol / L and 10μmol / L NaAsO 2 for 2h. The expression of HO-1 mRNA in 6h was significantly higher than that in control group and 2h exposure group (P <0.01). The mRNA expression of HO-1 mRNA at 24h and 24h after exposure to 10μmol / LNaAsO_2 was significantly higher than that at 6h exposure (P <0.01). However, HO-1 mRNA expression was not significantly increased at 24h. NaAsO2-induced HO-1 protein expression began at 6h, significantly higher than 6h in 12h group, 12h in 24h group (all P <0.01), and 5 and 10μmol / L of NaAsO2 were exposed for 6 and 12h And HO-1 protein expression at 24 h were 2.80, 9.34, 18.15 and 3.97, 12.92, 23.29 times that of the control group respectively. In addition, the HO-1 mRNA and protein expression of 10μmol / L NaAsO 2 exposed 12h and 24h were significantly higher than the corresponding time Of 5μmol / L group (P <0.01). [Conclusion] NaAsO2 exposure can effectively and consistently induce the mRNA and protein expression of HO-1 in Chang hepatocyte cell line, which is a cytoprotective adaptive response to inorganic arsenic exposure.