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使用插线法，建立大鼠右大脑中动脉急性栓塞（R－MCAO）的动物模型，检测了假手术组及R－MCAO后不同时间缺血局部及对侧相应脑组织的ET水平、NOS活性，血浆ET、NO浓度，结果缺血20min时，局部NOS活性即显著升高（P＜0．01），30min达高峰，然后明显下降；ET在缺血后30min开始持续升高（P＜0．05）；血浆NO水平在缺血30min时也出现了一过性的上升（P＜O．01），但缺血对侧脑组织NOS活性、ET水平无明显改变，且血浆ET水平亦无改变（P＞0．05）。表明，缺血局部ET－NO的失衡可能是导致局部脑组织损伤的重要机制之一。而血浆的ET、NO水平并不能代表即时的局部变化。
The animal models of acute right middle cerebral artery occlusion (R-MCAO) were established by using the patch-line method. The levels of ET and NOS in brain tissues of the ischemic and contralateral brain were measured at different time points after sham operation and R-MCAO (P <0.01), peaked at 30 min and then decreased significantly. ET began to increase at 30 min after ischemia (P <0, P <0.01) .05). Plasma NO levels also increased transiently at 30min after ischemia (P <0.01), but there was no significant change in NOS activity and ET level in the contralateral brain, and plasma ET level Change (P> 0.05). It shows that the imbalance of ET-NO in ischemic area may be one of the important mechanisms leading to local brain injury. Plasma ET and NO levels do not represent immediate local changes.