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目的探讨在可促进增殖范围内的压力加载与膀胱平滑肌细胞损伤的关系。方法将体外培养的膀胱平滑肌细胞分为实验组和对照组,实验组施予40 cm H2O(1 cm H2O=0.098 k Pa)压力加载培养,对照组无压力加载培养,24 h后观察细胞形态并采用免疫荧光检测膀胱平滑肌细胞骨架α-actin及碘化丙啶(PI)染色。结果实验组与对照组细胞形态结构无明显差异;α-actin免疫荧光面积测量实验组为(50.93±1.99)%,对照组为(24.70±1.61)%,两组间的表达量差异有统计学意义(t=32.404,P<0.001);PI染色阳性细胞数量实验组为(9.00±1.41)%,对照组为(3.50±2.12)%,实验组明显高于对照组,差异有统计学意义(t=6.110,P<0.001)。结论压力加载条件在促进细胞增殖的同时也会对膀胱平滑肌细胞造成损伤。
Objective To explore the relationship between pressure loading and bladder smooth muscle cell injury in a range of promoting proliferation. Methods The bladder smooth muscle cells cultured in vitro were divided into experimental group and control group. The experimental group was loaded with 40 cm H2O (1 cm H2O = 0.098 kPa) and the control group was cultured under no pressure. After 24 h, Immunofluorescence was used to detect the α-actin and propidium iodide (PI) staining of bladder smooth muscle cells. Results There was no significant difference between the experimental group and the control group. The immunofluorescence area of the experimental group was (50.93 ± 1.99)% in the experimental group and (24.70 ± 1.61)% in the control group, and the difference was statistically significant (9.00 ± 1.41)% in the experimental group and (3.50 ± 2.12)% in the control group, the experimental group was significantly higher than the control group (t = 32.404, P <0.001) t = 6.110, P <0.001). Conclusion The conditions of pressure loading can promote the cell proliferation as well as the injury of bladder smooth muscle cells.