论文部分内容阅读
目的:了解实验药物没食子鞣质及有效提取物对口腔细菌生物膜整体生物量的影响。方法:将附着于材料表面的细菌生物膜作为一个整体,运用半定量可见光测定OD值的方法,研究和评估实验药物没食子鞣质及有效提取物对口腔细菌生物膜生物密度的影响。结果:口腔细菌生物膜生长在体外表现出缓慢的非线性生长趋势,48~72 h出现一个较为稳定的平台期。没食子鞣质及有效提取物对细菌生物膜都有一定的浓度依赖性,其作用随着药物浓度提高而明显,药物对12 h细菌生物膜的影响最有效,对48 h成熟生物膜作用最小。结论:没食子鞣质及有效提取物能够减低口腔细菌生物膜在材料表面的整体生物量。
OBJECTIVE: To investigate the effects of the experimental drug gallotannin and the effective extract on the overall biomass of oral bacterial biofilm. Methods: Bacterial biofilm attached to the surface of the material as a whole, the use of semi-quantitative visible light determination of OD values to study and evaluate the experimental drug gallnut tannin and effective extract on oral bacterial biofilm biological density. Results: The growth of oral bacterial biofilm showed a slow non-linear growth trend in vitro, and a stable plateau appeared from 48 to 72 h. The content of tannin and the effective extract of the gallbladder had a certain concentration dependence on the bacterial biofilm. The effect was obvious with the increase of the concentration of the drug. The effect of the drug on the bacterial biofilm at 12 h and the effect on the mature biofilm at 48 h were the smallest. Conclusion: Gallic tannin and effective extract can reduce the overall biomass of oral bacterial biofilm on the material surface.