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运用细胞匀浆 MDA比色法 (体外法 )测定大鼠肝脏脂质过氧 ( LPO) ,研究铝、锌、钙分别作用 ,以及锌、钙与铝的分别协同作用对大鼠肝脏脂质过氧化物的影响。结果显示 ,铝加入量在0 .1 mmol~ 0 .1 nmol之间铝对肝脏 LPO影响较显著 ,与对照组比较 P<0 .0 1 ,且高浓度起抑制作用 ,低浓度起诱导作用。锌、钙加入量在 0 .0 1 mmol~ 0 .1μmol之间可以降低大鼠肝脏脂质过氧化代谢产物 ,并且两者都出现最大抑制峰。锌、钙可以降低铝所致肝脏 LPO值升高 ,与对照组比较 P<0 .0 1 ,说明锌、钙对铝有拮抗作用
The lipid peroxidation (LPO) in rat liver was measured by cell homogenate MDA assay (in vitro method), and the effects of aluminum, zinc and calcium were studied respectively. The synergistic effects of zinc, calcium and aluminum on rat liver lipid Oxide effects. The results showed that aluminum had a significant effect on hepatic LPO when the amount of aluminum was added between 0.1 mmol and 0.1 nmol, P <0.01 compared with the control group, and inhibited by high concentration and induced by low concentration. The addition of zinc and calcium between 0.1 mmol and 0.1 μmol could decrease the hepatic lipid peroxidation metabolites, and both showed the maximal inhibitory peak. Zinc and calcium can reduce the liver LPO caused by aluminum increased, compared with the control group P <0 01, indicating that zinc and calcium antagonistic effect on aluminum