为了探讨ＨＢＶ与肝细胞癌（ＨＣＣ）发病的关系，作者应用地高辛标记ＨＢＶＤＮＡ探针原位杂交及免疫组化检测了８０例ＨＣＣ组织及癌周肝组织的ＨＢＶＤＮＡ、ＨＢｓＡｇ。结果显示，癌周肝组织ＨＢＶ阳性６３例（７９％），癌组织ＨＢＶ阳性４９例（６０％），癌周阳性细胞数明显多于癌内。癌周ＨＢＶＤＮＡ，以复制型为主，而癌组织的ＨＢＶＤＮＡ大多属整合型。结果表明大多数ＨＣＣ病例与ＨＢＶ存在密切相关，而且整合型ＨＢＶＤＮＡ在ＨＣＣ发生过程中有着重要的作用。 To investigate the relationship between HBV and the development of hepatocellular carcinoma (HCC), the authors used in situ hybridization with digoxigenin-labeled HBV DNA probes and immunohistochemistry to detect HBV DNA and HBsAg in 80 HCC tissues and liver tissue around the cancer. The results showed that 63 cases (79%) were positive for HBV in the liver tissue, 49 cases (60%) were positive for HBV in the cancer tissue, and the number of positive cells in the cancer tissue was significantly higher than that in the cancer. Pericar- cinal HBV DNA is predominantly replicative, whereas cancerous tissues are mostly integrative. The results showed that most cases of HCC are closely related to the presence of HBV, and integrated HBV DNA plays an important role in the development of HCC.