【摘 要】
:
The aquatic grass Zizania latifolia grows symbiotically with the fungus Ustilago esculenta producing swollen structures called Jiaobai, widely cultivated in China. A new disease of Z. latifolia was found in Zhejiang Province, China. Initial lesions appear
【机 构】
:
Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects,Institute of Biot
【出 处】
:
浙江大学学报(英文版)(B辑:生物医学和生物技术)
论文部分内容阅读
The aquatic grass Zizania latifolia grows symbiotically with the fungus Ustilago esculenta producing swollen structures called Jiaobai, widely cultivated in China. A new disease of Z. latifolia was found in Zhejiang Province, China. Initial lesions appeared on the leaf sheaths or sometimes on the leaves near the leaf sheaths. The lesions extended along the axis of the leaf shoots and formed long brown to dark brown streaks from the leaf sheath to the leaf, causing sheath rot and death of entire leaves on young plants. The pathogen was isolated and identified as the bacterium Pantoea ananatis, based on 16S ribosomal RNA (rRNA) gene sequencing, multilocus sequence analysis (atpD (β-subunit of ATP synthase F1), gyrB (DNA gyrase subunit B), infB (translation initiation factor 2), and rpoB (β-subunit of RNA polymerase) genes), and pathogenicity tests. Ultrastructural observations using scanning electron microscopy revealed that the bacterial cells colonized the vascular tissues in leaf sheaths, forming biofilms on the inner surface of vessel walls, and extended between vessel elements via the perforated plates. To achieve efficient detection and diagnosis of P. ananatis, species-specific primer pairs were designed and validated by testing closely related and unrelated species and diseased tissues of Z. latifolia. This is the first report of bacterial sheath rot disease of Z. latifolia caused by P. ananatis in China.
其他文献
Acetaminophen, also known as N-acetyl-p-aminophenol (APAP), is commonly used as an antipyretic and analgesic agent. APAP overdose can induce hepatic toxicity, known as acetaminophen-induced liver injury (AILI). However, therapeutic doses of APAP can also
Plant metabolites are important for plant development and human health. Plants of celery (Apium graveolens L.) with different-colored petioles have been formed in the course of long-term evolution. However, the composition, content distribution, and mecha
目的 探讨1例具有腺和神经内分泌双向分化伴Peget样扩散的肛门癌合并腺癌的临床病理特征、组织学起源、诊断及鉴别诊断.方法 应用HE染色、免疫组织化学染色对重庆市中医院收集的1例肛门具有腺和神经内分泌双向分化的癌伴Paget样扩散合并腺癌进行临床病理分析,并进行相关的国内外文献复习,探讨其临床病理特征.结果 手术切除标本镜下形态:第一个肿物大部分瘤细胞呈弥漫性生长,核呈圆形或卵圆形,胞浆较少,局灶细胞可见核仁,胞质较丰富,可见印戒样细胞,肛门鳞状上皮内可见腺样分化细胞,胞浆空亮,核深染,有异型性.第二个肿
探讨慕课在人体组织学授课中的应用效果.选取185名2019级临床医学生随机分为研究组和对照组.研究组采用慕课+腾讯课堂线上直播的授课方式;对照组采用腾讯课堂线上直播的授课方式.通过应用“问卷星”单元测试、闭卷考试和问卷调查来分析将慕课引入组织学授课的教学效果.研究组学生的单元测试和期末考试成绩均显著高于对照组.研究组问卷调查结果显示,学生普遍认为慕课对组织学学习效果的提升有帮助.优质慕课资源作为组织学授课的补充,其教学效果和能力培养优于传统授课模式,值得推广和应用.
目的 探讨miR-21-5p对缺氧诱导的人肺动脉内皮细胞(human pulmonary artery endothelial cell,HPAEC)损伤的影响及其相关机制.方法 使用RT-qPCR检测在常氧和缺氧条件下HPAEC中miR-21-5p的表达水平.将miR-21-5p mimic转入HPAEC后,通过LDH活性分析法、Hoechst染色、Annexin V-FITC/PI染色和Cleaved caspase-3水平Western blot检测评估细胞损伤情况.通过生物信息学、荧光素酶活性法和
为了明确在运用环境磁学方法进行重金属污染评价时其所适用的土壤类型和磁学参数,对金华地区农田表层土壤进行了系统的环境磁学调查,分析南方地区土壤重金属污染的磁响应特征.结果表明:1)盆地内污染土壤的磁性突出表现为高磁化率值、高硬剩磁值及低的非磁滞剩磁磁化率与磁化率比值,与我国北方重污染土壤的磁性特征基本一致;2)水稻土表现为低磁化率值和极高的非磁滞剩磁磁化率与磁化率比值,与污染土壤磁学特征差别大,十分适合于使用环境磁学方法快速监测土壤重金属污染;3)山地丘陵土壤表现出较高的磁化率值和硬剩磁值,以及低的非磁滞剩
Cucurbitaceae is an important family of flower-ing plants containing multiple species of important food plants, such as melons, cucumbers, squashes, and pump-kins. However, a highly efficient genetic transforma-tion system has not been established for mos
为指导植物油在老化沥青再生中的合理应用,揭示植物油再生剂对老化沥青的再生及二次老化机制,探究其流变特性的变化,采用微、宏观相结合的方法对植物油再生沥青的微观结构组成、微观形貌特征和流变特性展开研究.结果表明:植物油再生剂因其富含芳香分,减少了老化沥青中羰基(C=O)和亚砜基(S=O)的相对含量,降低了沥青质的团聚程度,增大了“蜂状结构”形貌表面粗糙程度,故可实现对老化沥青的再生.老化沥青经植物油再生后,高温永久变形增大,高温抗车辙能力降低,但经二次老化后均得以恢复.老化沥青再生及二次老化的微观结构形貌及流
为了探究南、北半球降水变化规律,利用CMIP5/PMIP3中多个模式模拟结果(7个全强迫模式的算术平均值)对过去千年2个特征暖期南、北半球降水的时空变化差异及其机制进行了研究分析.结果表明:1)中世纪气候异常期,北半球平均降水增长要高于南半球;2)现代暖期,南、北半球平均降水增长较为一致;3)中世纪气候异常期,南、北半球降水变化差异主要是受南、北半球海洋表面温度变化差异的影响;4)热带太平洋海区纬向海洋表面温度梯度的减弱削弱了Walker环流,促进了现代暖期南、北半球降水的均衡变化.研究结果为进一步认识和
磁流变研抛时采用圆柱形永磁铁作为磁场发生装置存在“边缘效应”缺陷,且研抛时不同直径区域上的研抛速度不一致,导致研抛时材料去除不均匀,研抛区域较小.为提高研抛效果,研制了新型的磁流变研抛工具,并进行了理论分析.使用条形永磁铁组合作为磁场发生装置,利用永磁铁的“边缘效应”与“叠加效应”构建了线形增强磁场;并使用等效磁荷法拟合研抛间隙-磁场强度曲线,根据不同研抛区域的研抛速度确定磁场强度,逆向求解研抛间隙,对永磁铁表面进行切割,构建径向均匀的Preston材料去除函数;最后,进行了磁流变研抛对比实验.结果显示,