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目的 研究神经元的γ-氨基丁酸 (GABA)合成、分解和分泌与癫痫发病的关系。方法 用致痫剂马桑内酯 (CL )和抗痫剂苯妥英钠 (PHT)处理培养大鼠神经元 ,用高效液相色谱仪 ,紫外分光光度计分别检测培养神经元内外的 GABA浓度和神经元内γ-氨基丁酸转氨酶 (GABA - T)活性。结果 与对照组比较 ,CL使神经元内GABA- T活性显著增强 (P<0 .0 5 ) ,对神经元内外 GABA浓度没有影响。与 CL处理组以 (10 0μmol/ L CL培养神经元 2 4h)比较 ,PHT使 CL致痫神经元内 GABA- T活性显著减弱 (P<0 .0 5 ) ,基本恢复到对照组水平。但神经元内外 GABA浓度仍相对稳定。结论 癫痫发病中 ,痫性神经元合成、分解 GABA增强 ,分泌 GABA相对稳定
Objective To study the relationship between the synthesis, decomposition and secretion of γ-aminobutyric acid (GABA) in neurons and the pathogenesis of epilepsy. Methods The neurons of rat were cultured with epileptogenic agent (CS) and anticonvulsant phenytoin sodium (PHT). The concentrations of GABA and neurons inside and outside neurons were detected by HPLC and UV spectrophotometer respectively. Element within γ-aminobutyrate transaminase (GABA - T) activity. Results Compared with the control group, CL significantly increased the activity of GABA-T in neurons (P <0.05), and had no effect on the concentration of GABA in neurons. Compared with CL group, PHT significantly decreased GABA-T activity in CL-induced epileptiform neurons (P <0.05), and recovered to the level of control group with PHT (24 h cultured in neurons at 10 μmol / L CL). However, GABA concentration inside and outside neurons is still relatively stable. Conclusions In epilepsy, the synthesis and decomposition of GABA in seizure neurons are relatively stable