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目的观察小檗碱对人肝癌Bel-7402细胞增殖抑制及诱导凋亡的作用。方法体外培养人肝癌Bel-7402细胞,台盼兰活细胞计数及集落形成抑制实验观察不同浓度小檗碱对Bel-7402细胞的增殖抑制作用,Hochest33258染色观察凋亡形态学变化,流式细胞仪分析细胞周期及凋亡率。结果小檗碱可显著抑制Bel-7402细胞生长,使集落形成能力明显下降,均呈时间、剂量依赖性。小檗碱处理后72h,Hochest33258染色可观察到细胞核边集、固缩,有明显凋亡小体形成,流式细胞仪检测出现明显Sub-G1峰。结论小檗碱可以抑制人肝癌Bel-7402细胞增殖,诱导细胞凋亡,小檗碱可能具有治疗人肝细胞癌的潜在应用价值。
Objective To observe the effect of berberine on the proliferation and apoptosis of human hepatocellular carcinoma Bel-7402 cells. Methods Bel-7402 cells were cultured in vitro, the viable cell count and the inhibition of colony formation of trypan blue were observed in different concentrations of berberine on the proliferation of Bel-7402 cells. Hochest33258 staining was used to observe the morphological changes of apoptotic cells. Flow cytometry Analysis of cell cycle and apoptosis rate. Results Berberine could significantly inhibit the growth of Bel-7402 cells and decrease the colony formation ability in a dose and time-dependent manner. 72h after berberine treatment, Hochest33258 staining observed nuclear edge set, shrinkage, significant apoptotic bodies formed, flow cytometry showed significant Sub-G1 peak. Conclusion Berberine can inhibit the proliferation of human hepatocellular carcinoma Bel-7402 cells and induce apoptosis. Berberine may be potentially useful in the treatment of human hepatocellular carcinoma.