在生物中 ,特别是在真核生物中 ,DNA甲基化已成为阐明正常和病理基因表达现象的重要机制 ,因而已成为当前分子生物学的研究热点之一。由之而产生的 DNA甲基化分析技术也有了长足的发展。目前至少已报道了三类 10种此类技术。而近几年迅速发展起来的以 HSO3-转化为基础的各种 DNA甲基化检测技术 ,因其具有简便可行、准确灵敏等诸多优点 ,最令人瞩目。本文仅就这些方法的原理、灵敏度和准确性以及应用作一简要的综述 In organisms, especially in eukaryotes, DNA methylation has become an important mechanism to clarify the expression of normal and pathological genes, and has become one of the hot topics in current molecular biology. The resulting DNA methylation analysis technology has also made great strides. At least three types of 10 of these technologies have been reported so far. In recent years, the rapid development of HSO3-based transformation of DNA methylation detection technology, because of its simple and feasible, accurate and many other advantages, the most eye-catching. This article provides a brief overview of the principles, sensitivity and accuracy of these methods and their applications