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利用去乙酰含量分别为68.3%,70.3%,85.3%和92.2%的4种壳聚糖与离子交联剂三聚磷酸盐及共价交联剂栀子素共交联作用,制备出具有一定机械强度的共交联圆珠,分别命名为CB-Ⅰ、CB-Ⅱ、CB-Ⅲ和CB-Ⅳ。测定这些圆珠的直径、交联程度、硬度与弹性,结果 CB-Ⅰ、CB-Ⅱ、CB-Ⅲ和CB-Ⅳ的粒径分别为2.13,2.30,2.64 mm和3.28 mm。测定壳聚糖圆珠交联前、后自由胺基含量,计算得到CB-Ⅰ、CB-Ⅱ、CB-Ⅲ和CB-Ⅳ的交联程度分别为49.57%,52.27%,66.93%和61.71%。圆珠的硬度和弹性随着去乙酰度的增大而减小。扫描电子显微镜照片显示:随着去乙酰度的提高,圆珠表面粗糙度增大,并且其内部结构逐渐从紧致的蜂窝状转变为松散的海绵状。选择差异性较大的CB-Ⅰ、CB-Ⅲ和CB-Ⅳ3种圆珠于25℃,初质量浓度为0.2 mg/m L的条件下对胰蛋白酶抑制剂进行吸附,结果表明CB-Ⅲ具有最佳的吸附效果。
Four kinds of chitosan with deacetylated content of 68.3%, 70.3%, 85.3% and 92.2% were co-crosslinked with tripolyphosphate and covalent cross-linking agent, respectively. Mechanical strength of the cross-linked beads, respectively, named CB-Ⅰ, CB-Ⅱ, CB-Ⅲ and CB-Ⅳ. The diameter, degree of crosslinking, hardness and elasticity of these beads were measured. Results The particle sizes of CB-Ⅰ, CB-Ⅱ, CB-Ⅲ and CB-Ⅳ were 2.13, 2.30, 2.64 and 3.28 mm, respectively. The content of free amino groups before and after cross-linking of chitosan beads was measured, and the degree of cross-linking of CB-Ⅰ, CB-Ⅱ, CB-Ⅲ and CB-Ⅳ was 49.57%, 52.27%, 66.93% and 61.71% . The hardness and elasticity of the beads decrease as the degree of deacetylation increases. Scanning electron micrographs show that as the degree of deacetylation increases, the surface roughness of the beads increases, and its internal structure gradually changes from a compact honeycomb to a loose sponge. Trypsin inhibitor was adsorbed on CB-Ⅰ, CB-Ⅲ and CB-Ⅳ beads with different variability at 25 ℃ and initial concentration of 0.2 mg / m L. The results showed that CB- The best adsorption effect.