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以静电吸附力将多聚赖氨酸(PLL)自组装于玻璃基底上,制备了荷正电的PLL膜。研究了溶液p H、自组装时间、浓度等对成膜质量和吸附细菌性能的影响。研究表明,p H=7.4、时间为45 min、浓度为0.200 mg/m L为最佳组装条件。以大肠杆菌和金黄色葡萄球菌作为供试菌,通过涂板计数和显微镜计数法测试了荷正电膜对细菌的吸附能力。结果表明,在最佳条件下制备的PLL膜在30 min内对大肠杆菌吸附率达到92.3%,在45 min内对金黄色葡萄球菌的吸附率达到83.0%,其对革兰阴性菌的吸附率略大于对革兰阳性菌的吸附率。
The poly-lysine (PLL) was self-assembled on a glass substrate by electrostatic adsorption, and a positively charged PLL film was prepared. The effects of solution pH, self-assembly time and concentration on the quality of film formation and the properties of adsorbed bacteria were studied. Studies have shown that p H = 7.4, the time of 45 min, the concentration of 0.200 mg / m L for the best assembly conditions. Escherichia coli and Staphylococcus aureus were used as the test bacteria, and the adsorption capacity of the charged positive membrane to bacteria was tested by coating count and microscope counting. The results showed that the adsorption rate of E.coli reached 92.3% within 30 min and that of Staphylococcus aureus reached 83.0% within 45 min. The adsorption rate of Gram-negative bacteria Slightly greater than the gram-positive bacteria adsorption rate.