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目的探讨丁型肝炎病人肝组织中 HDAg、HDVRNA与 HBVDNA表达及关系。方法应用免疫组化和原位杂交技术检测了 79例丁型肝炎病人肝组织 HDAg、HDVRAN、HBVDNA表达 ,以 5 2例乙型肝炎病人肝组织 HBVDNA表达作对照。结果丁型肝炎 HBVDNA检出率 ( 2 7% )低于乙型肝炎 ( 44 % ) ( P<0 .0 5 )。在坏死灶边缘肝细胞和气球样变肝细胞浆内有大量的 HDVRNA蓄积或 HDAg呈浆型强表达 ,HDAg与 HDVRNA表达及分布呈一致性 ( P>0 .0 5 )。HBVDNA与 HDAg表达强度呈负性相关 ( P<0 .0 5 )。结论 HDV感染会抑制 HBV复制 ,尤其是 HDAg或 HDVRNA强表达时 ;在 HDV致病机制中可能既有 HDV的直接细胞毒性作用 ,也有 HBV和 HDV的协同作用
Objective To investigate the expression of HDAg, HDVRNA and HBVDNA in liver of patients with hepatitis D and its relationship. Methods The expression of HDAg, HDVRAN and HBVDNA in liver tissues of 79 patients with hepatitis D were detected by immunohistochemistry and in situ hybridization. The expression of HBVDNA in liver tissues of 52 patients with hepatitis B was compared. Results The detection rate of hepatitis B virus (HBVDNA) was lower than that of hepatitis B (27%) (44%) (P <0.05). A large number of HDV RNA accumulations or HDAg was strongly expressed in the plasma of hepatocytes and balloon-like hepatocytes in the margins of necrotic lesions, and the expression and distribution of HDAg and HDV were consistent (P> 0.05). HBVDNA was negatively correlated with HDAg expression (P <0.05). Conclusions HDV infection inhibits the replication of HBV, especially when HDAg or HDV RNA is strongly expressed. In the pathogenic mechanism of HDV, HDV may have both direct cytotoxicity and synergistic effects of HBV and HDV