China is one of the most important rapeseed producing countries in the world. Effective mechanical harvesting time for decreasing harvesting loss of winter oilseed rape has been becoming a critical factor. An elite cultivar Zhongshuang 11 (Brassica napus L.) was employed in two rounds of field experiments from 2009 to 2011. Seeds were sown with machine, three combine harvesting times namely combine harvesting A, B, and C (CHA, CHB, and CHC) were designed and manual harvesting (MH) as control was performed at maturity. The harvesting treatments were determined according to color of pod and seed in the field. Seed yield loss and quality in different treatments were evaluated. Results showed that both seed yields and harvesting losses in 2009-2010 were higher than that in 2010-2011, whereas seed oil contents in 2010-2011 were higher than that in 2009-2010. The highest yield appeared in CHB, which was significantly higher than that in MH. Furthermore, harvesting loss in CHB were 50% that in MH. Seed oil content and chlorophyll exhibited no obvious difference between CHB and MH. Economic profit analysis demonstrated that mechanical sowing/combine harvesting (MS/CH) showed an input/output ratio of 1:1.6, and it was 1:1.2 in mechanical sowing/manual harvesting (MS/MH). Labor-cost accounted for more than 70% of the total cost in MS/MH, which led to low profitability to a great extent. Our results suggested that CHB was the optimum harvesting time for winter oilseed rape along the Yangtze River. China is one of the most important rapeseed producing countries in the world. Effective mechanical harvesting time for decreasing harvesting loss of winter oilseed rape has been a critical factor. An elite cultivar Zhongshuang 11 (Brassica napus L.) was employed in two rounds of field experiments from 2009 to 2011. Seeds were sown with machine, three combine harvesting times of combine harvesting A, B, and C (CHA, CHB, and CHC) were designed and manual harvesting (MH) as control was performed at maturity. The harvesting treatments were determined according to color of pod and seed in the field. Seed yield loss and quality in different treatments were evaluated. Results showed that both seed yield and harvesting losses in 2009-2010 were higher than that in 2010-2011, The highest yield was in CHB, which was significantly higher than that in MH. Furthermore, harvesting loss in CHB were 50% that i Economic MHL analysis demonstrated that mechanical sowing / harvesting harvesting (MS / CH) showed an input / output ratio of 1: 1.6, and it was 1: 1.2 in Labor-cost accounted for more than 70% of the total cost in MS / MH, which led to low profitability to a great extent. Our results suggested that CHB was the optimum harvesting time for winter oilseed rape along the Yangtze River.