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一、试验目的:探索用红四氮唑测定大麦种子生活力的判断标准。二、材料和方法:取具有不同发芽率的“沪麦4号”、“77—130”、“沪辐48”等大麦种子,用30℃温水恒温浸种4小时或于20℃左右的室温下浸种16小时,使种子软化,用解剖刀沿胚部正中将种子纵切两半,一半放入染色板小孔内,以0.2%四唑溶液在25℃左右室温下染色5～6小时,另一半对应地放入以棉花做发芽床的染色板内,在25℃左右的室温下经24小时,使其萌动,逐粒对照,观察发芽种子与染色种子的情况,判断种子是否有生活力的染色标准。三、试验结果:试验共做十一次,计种子550粒,因染色失败或发芽不好等原因,染上色又发芽
First, the purpose of the experiment: To explore the determination of viability of barley seed with red tetrazolium criteria. MATERIALS AND METHODS: Barley seeds such as “Shanghai Wheat 4”, “77-130” and “Shanghai Spoke 48” with different germination rate were soaked in 30 ℃ warm water for 4 hours or at room temperature of 20 ℃ Seed soaking for 16 hours to soften the seeds, with a scalpel along the embryo in the middle of the seed longitudinal cut in half, half into the staining plate hole to 0.2% tetrazolium solution at 25 ℃ for about 5-6 hours at room temperature, the other Half corresponding to cotton germination bed into the staining plate, at room temperature at about 25 ℃ for 24 hours to germinate, the grain by contrast, the observation of germinated seeds and stained seeds, to determine whether the seeds have viability Dyeing standards. Third, the test results: The experiment a total of eleven times, totaling 550 seeds, due to staining failure or poor germination and other reasons, stained color and germination