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The mechanisms by which LH-RH or its agonists inhibit decidua formation and terminatepregnancy in rats are not clear. Evidence so far accumulated indicates that the gonadotropicreleasing hormone or its analogues may act directly on the uterus. If this is the case, then wewould be able to identify LH-RH receptors in the endometrial tissue. The results presentedhere demonstrate that the homogenate preparation of rat endometrial tissue bound ~125I-LH-RHwith an affinity constant of Ka = 1.3×10~8M~(-1). The binding capacity was measured as 236fmole/mg protein. It was found that the binding is highly specific, because no binding wasobserved in a variety of non-target tissues, such as liver, kidney and muscle. Competitive bind-ing with TRH, P substance and bradykinin did not occur under our experimental condition.It was of particular interest to note that Day 3 of pregnancy is a critical time showing a higherbinding activity. The significance of work is discussed.
The mechanisms by which LH-RH or its agonists inhibit decidua formation and terminatepregnancy in rats are not clear. Evidence so far accumulated that that gonadotropicreleasing hormone or its analogues may act directly on the uterus. If this is the case, then we can be able to identify LH-RH receptors in the endometrial tissue. The results presentedhereiform that the homogenate preparation of rat endometrial tissue bound ~125I-LH-RHwith an affinity constant of Ka = 1.3 × 10 ~ 8M -1. The binding capacity was was that the binding is highly specific, because no binding wasobserved in a variety of non-target tissues, such as liver, kidney and muscle. Competitive bind-ing with TRH, P substance and bradykinin did not occur under our experimental condition. It was of particular interest to note that Day 3 of pregnancy is a critical time showing a higher binding activity. The significance of work is discussed.