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从我省主要烟区烟草花叶病标样中所分离的毒源,在鉴别寄主苋色藜上形成局部枯斑,在心叶烟上则为系统侵染。病毒致死温度65～70℃,体外保毒期2～3天,稀释终点为1:1000～2000。电镜观察病毒粒体为球形和血清学试验不与烟草花叶病毒和芜菁花叶病毒起反应。根据这些特性,初步认为属典型的黄瓜花叶病毒(Cucumber Mosaic Virus)。本文用反向间接血凝反应、皂土凝聚反应、琼脂双扩散反应、试管沉淀反应和玻片凝聚反应对黄瓜花叶病毒进行了检测比较试验,其中以反向间接血凝法灵敏度最高,病株抽提液稀释至三万倍仍可检出,敏感度比琼脂双扩散试验高256倍,其诊断效率亦较好,均比其它测定法优越。
The source of the virus isolated from the tobacco mosaic disease standard sample in the main tobacco-growing area of our province formed a local spot on the differential host amaranth, while the systemic infection on the heart-leaf tobacco. Virus lethal temperature 65 ~ 70 ℃, in vitro protection of 2 to 3 days, the end of the dilution of 1: 1000 ~ 2000. Electron microscopy showed that the mitochondria were spherical and serological tests did not react with tobacco mosaic virus and turnip mosaic virus. According to these characteristics, initially considered a typical cucumber mosaic virus (Cucumber Mosaic Virus). In this paper, cucumber mosaic virus (CMV) was tested by reverse indirect hemagglutination, bentonite agglutination, agar double diffusion, tube sedimentation and glass slide agglutination. Among them, the reverse indirect hemagglutination assay showed the highest sensitivity Strain extract diluted to 30,000 times still detectable, sensitivity 256 times higher than the agar double diffusion test, the diagnostic efficiency is better, are superior to other assay.