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为了解中华甲虫蒲螨Pyemotes zhonghuajia Yu and Zhang对双条杉天牛Semanotus bifasciatus(Motschulsky)的致死过程,将已发育成熟的中华甲虫蒲螨膨腹体移入放有双条杉天牛2龄幼虫的玻璃指形管中,在蒲螨的一个生活史60h内,观察其取食行为,并在移入后每隔12h用考马斯亮蓝法分别测定天牛幼虫和蒲螨血淋巴中总蛋白质含量。结果显示,蒲螨蛋白含量总体呈现上升的趋势,在36～48h上升幅度最大,达61.78%;而天牛幼虫血淋巴总蛋白质含量则呈总体下降趋势,与对照相比,在36～48h之间下降最为明显;蒲螨总蛋白质含量在24h后处理组与对照组差异均为极显著,天牛幼虫总蛋白质含量在48h后处理组与对照组差异显著,这表明蒲螨在24h已搜寻到寄主开始取食,且取食效果明显,而天牛幼虫在48h后表现为麻痹致死,36～48h蒲螨取食对天牛致死起关键作用。12h时处理组的双条杉天牛幼虫总蛋白质含量比对照组略高,推测是由于幼虫对外寄生物蒲螨具有一定的免疫防御反应。
In order to understand the lethal process of Pyemotes zhonghuajia Yu and Zhang on the Semanotus bifasciatus (Motschulsky), the developed Chinese beetle pupae was transferred into the second instar larvae In the glass finger tube, the feeding behavior was observed within 60h in a life history of Po mite, and the total protein content in the haemolymph of Paecilomyces larvae and Polar bear were determined by Coomassie brilliant blue method every 12 hours after being transplanted. The results showed that the protein content of poplar mites increased in the whole period from 36 to 48 hours, up to 61.78%, while the total protein content of the haemolymph in the days larvae showed an overall downward trend. Compared with the control, Between the treatment group and the control group, the difference was significant (P <0.05). The total protein content of the pod mite after 24 hours was significantly different from that of the control group The host began to feed, and the feeding effect is obvious, while the day larvae showed paralysis after 48h death, 36 ~ 48h Po mite feeding on the death of the hormones played a key role. At 12h, the total protein content of Larvae lanceolata in the treatment group was slightly higher than that in the control group, presumably because larvae had certain immune defensive responses to the ectoparasite mite.