论文部分内容阅读
利用木瓜蛋白酶分别制备了抗汉坦病毒鼠源性单克隆抗体（ｍＡｂ）３Ｇ１和３Ｄ８Ｆ（ａｂ′）２片段。通过ＷａｔｅｒＤＥＡＥ４０ＨＲ阴离子交换色谱柱纯化后，非还原型ＳＤＳＰＡＧＥ呈现单一条带，相对分子质量（Ｍｒ）约１０００００。两种Ｆ（ａｂ′）２片段均具有良好的血凝抑制活性、病毒中和活性及对汉坦病毒感染乳鼠的保护作用
Anti-Hantavirus mouse monoclonal antibody (mAb) 3G1 and 3D8F (ab ’) 2 fragments were prepared by papain. After purification by WaterDEAE-40HR anion exchange chromatography, the non-reducing SDS-PAGE displayed a single band with molecular weight (Mr) of about 100,000. Both F (ab ’) 2 fragments have good hemagglutination inhibition activity, virus neutralization activity and protective effect on hantavirus infected neonatal rats