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为了解家猪在流行性出血热(EHF)疫区的流行病学意义,1986~1994年用反向间接血凝法(RPHA)、血凝抑制法(RPHI)、酶标葡萄球菌A蛋白(HPR-SPA)、间接免疫荧光法(IFAT)和反转录-多聚酶链反应(RT-PCR)对家猪与EHF的关系进行了现场调查和实验研究。结果证明:家猪可自然和实验感染EHF病毒;感染后病毒可经血液波及多个脏器;部分脏器有一过性病理改变;EHF病毒可在体内增殖并随排泄物排出体外。这些结果表明家猪可能作为EHF的宿主动物,具有一定的重要意义。
In order to understand the epidemiological significance of domestic pigs in the epidemic area of epidemic hemorrhagic fever (EHF), the reverse indirect hemagglutination (RPHA), hemagglutination inhibition (RPHI) and staphylococcal protein A HPR-SPA), indirect immunofluorescence (IFAT) and reverse transcription-polymerase chain reaction (RT-PCR) were used to investigate the relationship between domestic pigs and EHF. The results show that domestic pigs can naturally and experimentally infect EHF virus; after infection, the virus can affect multiple organs through blood; some organs have a transient pathological change; EHF virus can proliferate in vivo and excreted with the excrement. These results indicate that domestic pigs may be host animals for EHF, which is of great significance.