论文部分内容阅读
近年来，一氧化氮（NO）在败血症、感染性休克发病机理中的作用和地位得到日益重视。本文采用镉还原柱层析和比色法测定正常新生儿和新生儿败血症患儿的外周血亚硝酸/硝酸根离子（NO_2~-/NO_3~-）水平，同时在10日龄新生大白鼠绿脓杆菌败血症模型上进行验证，以探讨NO与新生儿败血症的关系。结果表明：20例新生儿败血症患儿的外周血NO_2~-/NO_3~-水平（263.04±300.01umol/L）显著高于30例正常新生儿（34.97±13.40umol/L）；外周血NO_2~-/NO_3~-水平与外周血肿瘤坏死因子（TNF）水平密切相关（r_3=0.6207）；8例外周血NO_2~-/NO_3~-水平高于200umol/L者，6例并发感染性休克，而12例外周血NO_2~-/NO_3~-
In recent years, the role and status of nitric oxide (NO) in the pathogenesis of sepsis and septic shock have been increasingly emphasized. In this paper, cadmium reduction column chromatography and colorimetric determination of normal neonates and neonatal sepsis in children with peripheral blood nitrite / nitrate ion (NO 2 - / NO 3 - -) levels, at the same time 10 days old neonatal rat green To verify the relationship between NO and neonatal sepsis. The results showed that the level of NO_2 ~ - / NO_3 ~ (263.04 ± 300.01umol / L) in 20 neonates with septicemia was significantly higher than that in 30 normal newborns (34.97 ± 13.40umol / L) - / NO_3 ~ - levels were closely related to tumor necrosis factor (TNF) level in peripheral blood (r_3 = 0.6207); 8 patients with peripheral blood NO_2 ~ - / NO_3- level higher than 200umol / L, 6 patients complicated with septic shock, While 12 cases of peripheral blood NO 2 - - / NO 3 -