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目的:探讨叶绿酸(CHL)联合赛特铂(JM216)对人SMMC-7721肝癌细胞凋亡及增殖的作用。方法:选用CHL(1×10-5 mol/L)及不同浓度JM216(10、20和40mg/L),1×10-5 mol/L的CHL分别联合不同浓度JM216作用于SMMC-7721肝癌细胞24、48和72h后,用MTT比色法观察其对肝癌细胞的生长抑制作用,并观察细胞形态变化;用流式细胞术分析药物作用48h时肝癌细胞的周期分布和凋亡情况。结果:CHL组、不同浓度JM216组和联合组细胞生长抑制率均高于对照组,联合组1和联合组2以及联合组3的细胞生长抑制率均高于相应浓度的JM216组。CHL组SMMC-7721细胞周期中G0/G1期比例为(55.94±3.49)%,高于对照组的(50.52±2.56)%,差异有统计学意义,P<0.05;而JM216组SMMC-7721细胞周期中S期比例为(45.12±1.42)%,高于对照组的(27.60±1.27)%,差异有统计学意义,P<0.05。CHL组和JM216组细胞凋亡率分别为(13.48±1.63)%和(15.51±2.09)%,均高于对照组的(1.61±0.47)%,差异有统计学意义,P<0.05;联合组细胞凋亡率为(30.22±3.20)%,高于JM216组和对照组,差异均有统计学意义,P<0.05。结论:CHL和JM216均可抑制肝癌SMMC-7721细胞的生长并诱导其凋亡,且联用后作用增强。
AIM: To investigate the effect of chlorophyllide (CHL) combined with sevoflurane (JM216) on the apoptosis and proliferation of human hepatocellular carcinoma cell line SMMC-7721. Methods: CHL (1 × 10-5 mol / L) and different concentrations of JM216 (10, 20 and 40 mg / L), 1 × 10-5 mol / L CHL were used in combination with different concentrations of JM216 on SMMC-7721 hepatoma cells After 24, 48 and 72 hours, MTT colorimetry was used to observe the growth inhibition effect on hepatocellular carcinoma cells. The morphological changes of cells were observed. The distribution and apoptosis of hepatoma cells were analyzed by flow cytometry at 48 hours. Results: The growth inhibition rates of CHL group, different concentrations of JM216 group and combination group were higher than that of control group. The cell growth inhibition rate of combination group 1 and combination group 2 and combination group 3 were higher than those of corresponding concentration of JM216 group. The percentage of G0 / G1 phase in SMMC-7721 cells in CHL group was (55.94 ± 3.49)%, which was significantly higher than that in control group (50.52 ± 2.56)%, P <0.05; The proportion of S phase in the cycle was (45.12 ± 1.42)%, which was higher than that in the control group (27.60 ± 1.27)%, the difference was statistically significant (P <0.05). The apoptosis rates in CHL group and JM216 group were (13.48 ± 1.63)% and (15.51 ± 2.09)%, respectively, which were significantly higher than those in control group (1.61 ± 0.47)%, P <0.05; The apoptosis rate was (30.22 ± 3.20)%, higher than that of JM216 group and control group, the difference was statistically significant, P <0.05. CONCLUSION: Both CHL and JM216 can inhibit the growth of hepatocellular carcinoma SMMC-7721 cells and induce their apoptosis, and their combined effects are enhanced.