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[目的]探讨乳腺癌组织和细胞系中RUNX3基因mRNA与蛋白表达及在乳腺癌发生中的作用。[方法]运用RT-PCR、WesternBlot方法研究5种乳腺癌细胞系、1种正常乳腺细胞系和30对新鲜乳腺癌及癌旁对照组织中RUNX3mRNA和蛋白表达。[结果]5种乳腺癌细胞系中有3种(T47D、MCF7和SKBR3)RUNX3mRNA和蛋白表达阴性。30例新鲜的乳腺癌组织中RUNX3mRNA和蛋白表达明显低于癌旁正常对照组织,差异具有统计学意义。RUNX3蛋白表达与乳腺癌临床分期、淋巴结转移相关,而与患者的年龄、病理分级无关。[结论]RUNX3基因可能作为一个抑癌基因参与乳腺癌的发生。
[Objective] To investigate the expression of RUNX3 mRNA and protein in breast cancer tissues and cell lines and its role in the development of breast cancer. [Method] The expression of RUNX3 mRNA and protein in 5 breast cancer cell lines, 1 normal breast cell line and 30 pairs of fresh breast cancer and adjacent normal tissues were studied by RT-PCR and Western Blot. [Results] Three of the five breast cancer cell lines (T47D, MCF7 and SKBR3) had negative RUNX3 mRNA and protein expression. The expression of RUNX3 mRNA and protein in 30 cases of fresh breast cancer tissues was significantly lower than that in adjacent normal tissues, the difference was statistically significant. RUNX3 protein expression and clinical stage of breast cancer, lymph node metastasis, but not with the patient’s age, pathological grade. [Conclusion] RUNX3 gene may participate in the occurrence of breast cancer as a tumor suppressor gene.