作者用Dulbecco改良的Eagle培养基(DMEM),加20%的马血清或胎牛血清培养小鼠骨髓,细胞浓度为1×10~6/毫升,把10毫升骨髓细胞悬液接种在25厘米~2的塑料组织培养瓶中,在37℃下,饱和湿度及含10% CO_2的空气条件下培养,每间隔一周吸出5毫升培养液,然后更换新配制的培养基和血清,2～3周则瓶壁长满骨髓细胞的贴壁细胞 The authors used Dulbecco’s modified Eagle’s medium (DMEM), plus 20% horse serum or fetal bovine serum culture mouse bone marrow cells at a concentration of 1 × 10 ~ 6 / ml, the 10 ml bone marrow cell suspension was inoculated in 25 cm ~ 2 plastic tissue culture flask and cultured at 37 ° C under saturated humidity and air with 10% CO 2, and 5 ml of culture solution was aspirated every other week, and then the newly prepared medium and serum were replaced. Bottle wall covered with adherent cells of bone marrow cells