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目的:考察乌参醒脑方(WSXN)对大鼠脑缺血致血管性痴呆的神经保护作用。方法:大鼠随机分为假手术组、模型组、WSXN低、中、高剂量组(28,56,112 mg·kg-1)和EGB761阳性对照组(80 mg.kg-1),每组10只;采用改良的Pulsinelli四血管阻断法制备全脑缺血-再灌注损伤学习记忆的大鼠血管性痴呆模型。灌胃给药20 d(预防6 d+治疗14 d),对照组给予等容量溶媒。采用Morris水迷宫定向航行试验及空间探索试验检测各组大鼠的学习记忆能力,光镜观察脑海马神经组织及细胞形态学变化。结果:大鼠全脑缺血再灌注引起脑海马神经细胞损伤和学习忆力能力损害;中、高剂量WSXN和EGB761能剂量依赖性地显著增加脑缺血后大鼠海马幸存的活锥体神经元密度和尼氏体数目,还能改善血管性痴呆大鼠的空间学习记忆和参照记忆能力。结论:乌参醒脑方对全脑缺血再灌注神经损伤和血管性痴呆有显著的治疗作用。
Objective: To investigate the neuroprotective effect of Wushen Xingnao Recipe (WSXN) on vascular dementia induced by cerebral ischemia in rats. Methods: The rats were randomly divided into sham operation group, model group, WSXN low, medium and high dose groups (28,56,112 mg · kg-1) and EGB761 positive control group (80 mg · kg-1) The vascular dementia model of rats with global cerebral ischemia-reperfusion injury learning and memory was established by modified Pulsinelli four-vessel occlusion method. Gavage 20 d (prevention 6 d + treatment 14 d), the control group was given the same volume of vehicle. Morris water maze directional navigation test and space exploration test were used to detect the learning and memory abilities of rats in each group. The morphological changes of hippocampal nerve tissue and cells were observed by light microscope. RESULTS: Cerebral ischemia-reperfusion in rats caused damage to the hippocampal neurons and impairment of learning and memory ability. Middle and high doses of WSXN and EGB761 significantly increased the survival of hippocampal neurons in live hippocampal neurons in a dose-dependent manner Element density and the number of Nissl, but also improve spatial learning and memory ability of vascular dementia rats. Conclusion: Wu Shen Xing Nao Prescription has a significant therapeutic effect on nerve injury and vascular dementia during global cerebral ischemia-reperfusion.