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目的:研究附子不同的临床使用剂量对小鼠心脏超氧化物歧化酶(SOD)活性、丙二醛(MDA)、一氧化氮(NO)含量的影响,探讨其对心脏的毒性机制方法:制备附子及与甘草配伍的水煎液,将小鼠随机分为9组,分别以附子15,45,75,105g的临床使用剂量按等效换算系数换算成小鼠给药卉剂量,给小鼠灌胃20d,测其心脏组织SOD活性及MDA、NO含量。结果:与对照组比较,单附子临床剂量45,75,105 g组小鼠心脏SOD活性明显增加,MDA含量显著降低(P<0.05)。甘草配伍后的各组与对照组比较,SOD含量有增加的趋势,但仅105 g组有明显差异(P<0.05);除15 g组外,其余各组MDA含量均显著降低(P<0.05);NO含量均呈下降趋势,但差异无统计学意义(P>0.05)。结论:大剂量使用附子对心脏的SOD活力、MDA和NO含量没有负面影响,相反能减轻小鼠心脏组织氧化反应。
Objective: To study the effect of different doses of aconite on the activity of SOD, the content of malondialdehyde (MDA) and the content of nitric oxide (NO) in the heart of mice and to explore its mechanism of toxicity to the heart. Methods: Aconite and licorice compatibility decoction, the mice were randomly divided into 9 groups, respectively, to the clinical use of aconite 15,45,75,105 g dose equivalent conversion factor equivalent to the mice to give the amount of floral give mice irrigation Twenty days after operation, the activity of SOD and the contents of MDA and NO in heart tissue were measured. Results: Compared with the control group, the SOD activity in the heart of the 45, 75 and 105 g single-acupuncture groups significantly increased and the MDA content decreased significantly (P <0.05). Compared with the control group, the content of SOD in licorice group increased, but only 105 g group had significant difference (P <0.05). In addition to 15 g group, the content of MDA in other groups were significantly decreased (P <0.05 ); NO content showed a downward trend, but the difference was not statistically significant (P> 0.05). Conclusion: The high dose of aconite had no negative effect on the activity of SOD, the content of MDA and NO in the heart, on the contrary it could reduce the cardiac tissue oxidation in mice.