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称取卵清蛋白置于盛有蒸馏水的1000ml高型烧杯中,用玻棒轻轻搅拌使之溶于水中(见表1)。若以水溶性添加剂代替配方中部分卵清蛋白,就要在加蛋白之前先溶于水。缓缓搅拌下,加入硝基呋喃妥因,使之在卵清蛋白溶液中分散均匀。再将含有0.6ml司盘85的液状石蜡(USP)250ml加人盛有硝基呋喃妥因混悬液的烧杯中,用三叶搅拌器不断搅拌。随后加速至约300转/分搅拌10分钟。置于50℃的水浴中加热5分钟。继续搅拌,以每分钟1～2℃的速度升温至75℃,维持10分钟。在该温度下卵清蛋白发生凝固,同时使硝基呋喃妥因微粒包含在卵清蛋白基质中。将冰块加入水浴中,使温度迅速
Ovalbumin was weighed and placed in a 1000 ml high-density beaker containing distilled water and gently agitated with a glass stick to dissolve in water (see Table 1). If the water-soluble additives to replace part of the ovalbumin, it is necessary to dissolve in water before adding protein. With gentle stirring, nitrofurantoin is added to disperse homogeneously in the ovalbumin solution. Then, 250 ml of liquid paraffin (USP) containing 0.6 ml Span 85 was added to a beaker containing a nitrofurantoin suspension and stirred continuously with a clover mixer. Then accelerate to about 300 rpm for 10 minutes. Place in a water bath at 50 ° C for 5 minutes. Stirring was continued and the temperature was raised to 75 ° C at a rate of 1 to 2 ° C per minute for 10 minutes. Ovalbumin coagulates at this temperature, while the nitrofurantoine particles are contained in the ovalbumin matrix. Add ice cubes to the water bath to allow rapid temperature