确定木瓜蛋白酶去除龙芽楤木多糖中蛋白质的最佳工艺。在单因素试验基础上,采用Box-Behnken试验设计优化酶用量、时间、温度的最佳条件。酶法脱蛋白最优工艺条件为酶用量2.7%、时间29.4 min、温度52.0℃,蛋白质脱除率为57.4%。酶法脱蛋白效果较好,操作简捷,便于工业化生产。 To determine the best papain to remove the protein from the sprout of Radix albicans polysaccharides. Based on the single factor experiment, the optimal conditions of enzyme dosage, time and temperature were designed by Box-Behnken test. The optimal enzymatic deproteinization conditions were as follows: enzyme dosage 2.7%, time 29.4 min, temperature 52.0 ℃, protein removal rate 57.4%. Enzymatic deproteinization is better, the operation is simple, easy to industrial production.