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目的研究人参皂甙Rb1体外对正常男性精子运动的影响,探讨其可能的作用机制。方法将18例正常男性经手淫获得的精子,通过上游优化法处理后,与10μmol/L人参皂甙Rb1共同孵育0．5h、1h、2h、3h、4h、5h,应用计算机辅助的精子分析系统(CASA),观察随时间延长人参皂甙Rb1对人精子各运动参数的作用。结果随着时间延长,精子活动能力均有不同程度下降；10μmol/L人参皂甙Rb1能明显提高正常男性精子的运动能力(A+B)%、精子存活率(Mot)、前向运动精子百分率、曲线运动速度(VCL)、直线运动速度(VSL)和平均路径速度(VAP),与空白对照组相比,差异均有显著性(P＜0．05)。结论随着时间延长,低浓度人参皂甙Rb1在体外能明显提高止常男性精子的存活率和运动能力,本研究为人参皂甙Rb1的临床应用提供了斯的实验基础。
Objective To investigate the effect of ginsenoside Rb1 on sperm motility in normal males and to explore its possible mechanism. Methods The spermatozoa obtained from masturbation in 18 normal males were treated with an upstream optimization method and incubated with 10 μmol/L ginsenoside Rb1 for 0.5 h, 1 h, 2 h, 3 h, 4 h, and 5 h. The computer-assisted sperm analysis system was used. CASA) Observe the effect of ginsenoside Rb1 on the motion parameters of human spermatozoa over time. Results The sperm motility decreased with time, and 10 μmol/L ginsenoside Rb1 significantly increased the sperm motility (A+B)%, sperm motility (Mot), and percentage of sperm in forward motility. Curvilinear velocity (VCL), linear velocity (VSL), and mean path velocity (VAP) were significantly different from those in the blank control group (P<0.05). Conclusion With the extension of time, low concentrations of ginsenoside Rb1 can significantly increase the survival rate and exercise capacity of only male spermatozoa in vitro. This study provides the experimental basis for the clinical application of ginsenoside Rb1.