本文拟针对在慢性粒细胞白血病发病中起关键作用的ｂｃｒａｂｌ 融合基因， 构建特异性的系列核酶载体。为此，我们针对ｂｃｒａｂｌ 融合位点设计、合成了３ 个相邻的锤头状核酶。通过基因重组将３ 个单核酶按顺序定向克隆入改建的ｐＧＥＭ３ｚｆ 载体中，构建了ｂｃｒａｂｌ 单核酶、双核酶及三核酶体外转录载体， 并在此基础上将三单位核酶克隆入ｐ ＤｏＲｎｅｏ 载体中构建三核酶逆转录病毒载体。此外， 通过ＰＣＲ 方法， 扩增了ｂｃｒａｂｌ 融合位点附近约３７６ｂｐ 的序列，成功地构建了ｂｃｒａｂｌ 融合基因体外转录载体。本文构建的ｂｃｒａｂｌ 特异性系列载体，为今后遴选特异、高效的核酶打下了基础，并为将核酶用于自体骨髓移植的体外骨髓净化创造了条件。 This article aims to build a specific series of ribozyme vectors for the bcrabl fusion gene that plays a key role in the pathogenesis of chronic myeloid leukemia. For this purpose, we designed and synthesized three adjacent hammerhead ribozymes for the bcr-abl fusion site. Three single-ribosomal enzymes were cloned into the reconstructed pGEM-3zf vector by gene recombination, and bcrabl single-ribozyme, dual-ribozyme and tri-nuclease in vitro transcription vectors were constructed, and on this basis, three units of nuclear were constructed. The enzyme was cloned into the pDoRneo vector to construct a trinuclease retroviral vector. In addition, the 376 bp sequence near the bcrabl fusion site was amplified by PCR and the bcrabl fusion gene in vitro transcriptional vector was successfully constructed. The bcrabl specific vector constructed in this study lays the foundation for the selection of specific and efficient ribozymes in the future, and creates conditions for the in vitro bone marrow purification using ribozymes for autologous bone marrow transplantation.