基因治疗的关键之一是载体,聚乙烯亚胺(PEI)是重要的肿瘤基因治疗转导载体,在非病毒载体的研究中有重要的地位。聚乙烯亚胺有较强的结合DNA和黏附细胞的能力,具有较高的转导效率。尽管在体外聚乙烯亚胺转染真核细胞取得了很大的进步,但应用于体内基因转导仍然面临着如解剖大小的限制等一系列障碍。 One of the key factors in gene therapy is carrier. Polyethylenimine (PEI) is an important transduction vector for tumor gene therapy and plays an important role in the study of non-viral vectors. Polyethyleneimine has a strong ability to bind DNA and adhere to cells, with high transduction efficiency. Although in vitro polyethyleneimine transfection of eukaryotic cells has made great progress, but used in vivo gene transduction is still faced with such as the size of the anatomical limitations and a series of obstacles.