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目的:建立同时测定罗布麻叶药材及其提取物中芦丁、金丝桃苷、异槲皮苷、紫云英苷、槲皮素和山柰酚含量的方法。方法:采用HPLC法,Shim pack ODS色谱柱(4.6 mm×250 mm,5μm),柱温40℃,以0.2%磷酸水溶液和0.2%磷酸乙腈溶液为流动相进行液相色谱梯度洗脱,检测波长360 nm,流速1.0 mL.min-1。结果:6种黄酮成分在40 min内均达到基线分离,线性关系良好(R2>0.999 7,n=6),日内、日间精密度RSD≤2.0%。该法成功地应用于罗布麻药材及其提取物中6种黄酮含量的测定,药材和提取物样品溶液中6种黄酮的平均回收率分别为97.30%～105.8%(RSD≤3.6%)和98.70%～103.1%(RSD≤2.2%)。结论:本方法操作简单、结果准确,具有较好的重复性和稳定性,为罗布麻叶药材及其提取物质量控制提供了一个很好的依据。
OBJECTIVE: To establish a method for the simultaneous determination of rutin, hyperoside, isoquercitrin, astragalin, quercetin and kaempferol in Apocynum leaves and its extracts. Methods: The HPLC method was applied on a Shim pack ODS column (4.6 mm × 250 mm, 5 μm) with a column temperature of 40 ℃. The mobile phase was eluted with 0.2% phosphoric acid solution and 0.2% 360 nm, flow rate 1.0 mL.min-1. Results: The flavonoids of 6 flavonoids all achieved baseline separation within 40 min. The linearity was good (R2> 0.999 7, n = 6). The intra-day and inter-day RSD was less than or equal to 2.0%. The method was successfully applied to the determination of six flavonoids in Apocynum venetum L. and its extracts. The average recoveries of six flavonoids in herbs and extracts were 97.30% -105.8% (RSD ≤ 3.6%) and 98.70 % ~ 103.1% (RSD≤2.2%). Conclusion: The method is simple and accurate with good repeatability and stability, which provides a good basis for the quality control of Apocynum venetum L. extract and its extracts.