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目的研究survivin反义寡核苷酸(ASODN)转染对肾透明细胞癌786O细胞的survivin蛋白表达、细胞凋亡、增殖的影响,及其对表阿霉素诱导细胞凋亡的作用。方法设计并合成特异性靶向survivin的ASODN及正义寡核苷酸(SODN),将其转入786O细胞。设空白对照组、脂质体对照组、SODN组和600nmol/LASODN组,处理24h后收获各组细胞。透射电子显微镜观察细胞形态变化,免疫组化法检测各组细胞survivin表达情况,流式细胞术检测各组细胞增殖和凋亡指数。结果ASODN组的细胞呈典型凋亡的形态学改变,而对照组细胞生长良好;ASODN组细胞survivin表达减弱,凋亡指数明显升高(P<0.05);转染ASODN24h后,表阿霉素诱导786O细胞凋亡的作用明显增强。结论survivinASODN能下调survivin蛋白表达,诱导肾透明细胞癌786O细胞凋亡,抑制786O细胞增殖,并增强表阿霉素诱导的细胞凋亡。
Objective To investigate the effect of survivin antisense oligonucleotide (ASODN) transfection on survivin protein expression, apoptosis and proliferation in renal clear cell carcinoma 786O cells and its effect on epirubicin-induced apoptosis. Methods Specific ASODN and sense oligonucleotide (SODN) targeting survivin were designed and synthesized and transferred into 786O cells. The blank control group, liposome control group, SODN group and 600nmol / L ASODN group were set up and the cells of each group were harvested after 24h treatment. Cell morphology was observed by transmission electron microscopy. Survivin expression in each group was detected by immunohistochemistry. Cell proliferation and apoptosis index were detected by flow cytometry. Results The cells in ASODN group showed morphological changes of typical apoptosis, while the cells in control group grew well. The expression of survivin in ASODN group was decreased and the apoptosis index was significantly increased (P <0.05). After induced by epirubicin for 24 hours, The role of 786O apoptosis was significantly enhanced. Conclusion Survivin ASODN can down-regulate the expression of survivin protein and induce the apoptosis of 786O cells, inhibit the proliferation of 786O cells and enhance the apoptosis induced by epirubicin.