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目的:观察正丁酸钠对肺纤维化大鼠高迁移率族蛋白B1(high mobility group box 1,HMGB1)和α平滑肌肌动蛋白(smooth muscle actin-α,α-SMA)表达的影响。方法:将30只大鼠随机分为对照组、模型组和治疗组,每组10只。3组大鼠用水合氯醛腹腔注射麻醉,竖立固定,经口气管插管,对照组气管内注入生理盐水,模型组和治疗组利用博莱霉素建立大鼠肺纤维化模型,治疗组多次静脉注射正丁酸钠,14d分离肺,将肺组织用甲醛固定,切片,行常规HE和Masson染色,镜下比较3组肺泡炎和肺纤维化程度;并行HMGB1和α-SMA免疫组织化学染色。结果:对照组大鼠肺组织结构完整,可见极少红色纤维,无α-SMA阳性表达,HMGB1少量阳性表达,模型组肺泡间隔内成纤维细胞增多,HMGB1和α-SMA蛋白表达较对照组明显增多(P<0.01),治疗组肺组织中HMGB1、α-SMA蛋白阳性染色细胞较对照组多,但较模型组明显减少,差异有统计学意义(P<0.01)。结论:在博莱霉素诱导的肺纤维化大鼠的肺组织中,HMGB1和α-SMA蛋白表达明显增加,且二者表达量明显正相关,正丁酸钠干预治疗后肺组织HMGB1表达受抑制,并抑制α-SMA的活化,对肺纤维化大鼠有治疗作用。
OBJECTIVE: To observe the effect of sodium butyrate on the expression of high mobility group box 1 (HMGB1) and α-smooth muscle actin-α (α-SMA) in rats with pulmonary fibrosis. Methods: Thirty rats were randomly divided into control group, model group and treatment group, with 10 rats in each group. The rats in the three groups were anesthetized by intraperitoneal injection of chloral hydrate, erected fixedly, intubated through the endotracheal tube, and injected with saline into the trachea of the control group. Bleomycin was used to establish the pulmonary fibrosis model in the model group and the treatment group. The rats were randomly divided into three groups: sodium hypobateutate (NS), intravenous injection of sodium butyrate (14) and pulmonary fixation (14 days). The lungs were fixed with formaldehyde and stained with HE and Masson. The levels of alveolitis and pulmonary fibrosis in the three groups were compared microscopically. HMGB1 and α-SMA immunohistochemistry dyeing. Results: The lung tissue of rats in the control group was intact with few red fibers, no α-SMA positive expression and a small amount of HMGB1 positive expression. The fibroblasts in the alveolar septum of the model group were increased, and the expressions of HMGB1 and α-SMA protein were significantly higher than those in the control group (P <0.01). Compared with the model group, the number of HMGB1 and α-SMA positive staining cells in the treatment group was significantly more than that in the control group, the difference was statistically significant (P <0.01). CONCLUSION: The expressions of HMGB1 and α-SMA in lung tissue of bleomycin-induced pulmonary fibrosis rats were significantly increased, and the expressions of HMGB1 and α-SMA were significantly positively correlated. The expression of HMGB1 in lung tissue after sodium butyrate intervention Inhibition, and inhibition of α-SMA activation, pulmonary fibrosis in rats have a therapeutic effect.