自从1959年Markert和Moller提出同工酶的概念以来,目前已知生物体内半数左右的酶种都有同工酶存在。同工酶是分子水平的指标;依据同工酶表现型可以比较直接地判断基因的存在与表达规律;同工酶分析简便,且不易受环境及人为因素的影响。正因为如此,近30年来,同工酶广泛应用于植物遗传 育种、分类及发育等众多领域中。在柑橘上,首先进行同工酶研究的学者是Wamer和Upadhya。他们研究了光周期对三个柑橘品种及一个枳品系的过氧化物酶(POX)同工酶的影响(1968),从此揭开 Since Markert and Moller proposed the concept of isozymes in 1959, it is known that about half of the isozymes present in the organism are isozymes present. Isoenzyme is an indicator of molecular level. According to the phenotype of isozymes, the existence and expression of genes can be directly judged. Isoenzyme analysis is simple and not easily affected by environmental and human factors. Because of this, in the recent 30 years, isozymes are widely used in many fields of plant genetic breeding, classification and development. On citrus, the first scholars studying isozymes are Wamer and Upadhya. They studied the effects of photoperiod on the peroxidase (POX) isozymes of three citrus varieties and one trifoliate orange (1968)