作者进行下列实验观察:1) 胆结石形态。85块病人结石经脱水固定后剖开,在扫描电镜下观察其表面及内部。2) 粘着于结石表面的细菌。取78块胆结石(色素结石及胆固醇结石各39块),从病人胆汁培养分离出15种细菌。将细菌放入灭菌的胆汁(42例)或肉汤(36例)中,做成15种不同细菌的悬浊液。在每种细菌悬浊液中放入一块已灭菌的结石,进行培养。每种 The authors conducted the following experimental observations: 1) gallstones morphology. 85 patients with stone after dehydration fixed cut open, observed under the scanning electron microscope surface and internal. 2) bacteria sticking to the stone surface. Take 78 gallstones (39 pigmented stones and cholesterol stones) and isolated 15 kinds of bacteria from the patient’s bile culture. Bacteria were placed in sterilized bile (42 cases) or broth (36 cases) and made into a suspension of 15 different bacteria. Place a sterilized stone in each bacterial suspension and incubate. Each kind