论文部分内容阅读
目的 探讨荧光原位杂交技术 (FISH)应用于绒毛及羊水间期细胞进行产前诊断的可行性。方法 应用 18、X号染色体着丝粒探针及 2 1号染色体特定区域探针 ,分别对绒毛及羊水间期细胞进行荧光原位杂交 ,并与中期分裂相进行比较分析。结果 绒毛及羊水间期细胞均出现了杂交信号 ,尤以绒毛细胞杂交比率高 ,信号强 ;常染色体探针出现 3个及 1个杂交点的比率约为 1%和6 % ,X染色体探针出现 3点的比例也在 1%左右。结论 荧光原位杂交技术对检出染色体非整倍体有较强的特异性 ,同时也为染色体非整倍体的检出提供了诊断依据。FISH技术应用于产前诊断具有快速、简便、灵敏的优点 ,有较大的临床应用价值
Objective To investigate the feasibility of fluorescence in situ hybridization (FISH) for prenatal diagnosis of villi and amniotic fluid cells. Methods Fluorescent in situ hybridization (ISH) was performed on villus and amniotic fluid mesenchymal cells, using 18, X chromosome centromeric probes and 21 specific probes. The results were compared with the metaphase. Results The hybridization signals were observed in the meiotic cells of villi and amniotic fluid. The hybridization rate of villus cells was high and the signal was strong. The ratio of 3 and 1 hybrids of autosomal probes was about 1% and 6%, respectively. X chromosome probes The ratio of 3:00 is also about 1%. Conclusion Fluorescence in situ hybridization (FISH) has strong specificity for detecting aneuploidy of chromosomes and also provides a diagnostic basis for detecting aneuploidy of chromosomes. FISH technology used in prenatal diagnosis with fast, simple and sensitive advantages, a greater clinical value