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目的研究转化生长因子β１（ＴＧＦβ１）ｍＲＮＡ在原发性肝细胞癌患者中的表达及其临床意义。方法用ＲＴ－ＰＣＲ加ＤｏｔＢｌｏｔ法检测原发性肝细胞癌患者肝组织和外周血单个核细胞（ＰＢＭＣ）中ＴＧＦ－β１ｍＲＮＡ水平，并以正常肝组织和正常人的ＰＢＭＣ为对照。结果ＴＧＦ－β１ｍＲＮＡ水平在原发性肝细胞癌患者组肝组织（２．２２±０．８４，ｎ＝１６）和ＰＢＭＣ中（１．８３±１．２，ｎ＝２５）比较，差异无显著性（Ｐ＞０．０５），但两者均高于正常肝组织（０．９４±０．７６，ｎ＝５）和正常人的ＰＢＭＣ（０．６２±０．４０，ｎ＝１６）水平。结论ＴＧＦ－β１ｍＲＮＡ水平与原发性肝细胞癌有关，ＰＢＭＣ中ＴＧＦ－β１ｍＲＮＡ检测可望作为一项代替肝组织活检的指标，其表达水平与肝癌有关。
Objective To investigate the expression of transforming growth factor β1 (TGFβ1) mRNA in primary hepatocellular carcinoma and its clinical significance. Methods The levels of TGF-β1 mRNA in hepatic tissue and peripheral blood mononuclear cells (PBMC) of patients with primary hepatocellular carcinoma were detected by RT-PCR and DotBlot method. The levels of PBMC in normal liver tissue and healthy controls were compared. Results There was no significant difference in TGF-β1 mRNA levels between liver tissue (2.22±0.84, n=16) and PBMC (1.83±1.2, n=25) in patients with hepatocellular carcinoma. (P>0.05), but both were higher than normal liver tissue (0.94±0.76, n=5) and normal human PBMC (0.62±0.40, n=16) levels . Conclusion The level of TGF-β1 mRNA is related to primary hepatocellular carcinoma. Detection of TGF-β1 mRNA in PBMC is expected to be an index to replace liver biopsy. Its expression level is related to hepatocellular carcinoma.