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目的：探讨表皮生长因子（EGF）在喉癌患者血清中的表达及其临床意义．方法：用放射免疫方法对38例喉癌患者手术前后的血清EGF进行检测．20例喉良性病变及30例健康成人为对照．同时用APA免疫组化法检测EGF在组织中的表达．结果：喉癌组血清中EGF阳性率73．7％．术前血清EGF水平显著高于喉良性病变组及健康对照组（P＜0．01）．术后血清EGF下降至接近正常．其中2例术后复发伴远处转移者血清EGF重新升高．高水平血清EGF与喉癌病理分级及淋巴结转移相关，与年龄、病变部位及临床分期无关．免疫组化EGF阳性表达为63．2％，与病理分级有关．结论：血清EGF可以做为喉癌诊断、预后和术后随访有用的生物标志．
Objective: To investigate the expression of epidermal growth factor (EGF) in serum of laryngeal cancer patients and its clinical significance. Methods: Serum EGF levels in 38 patients with laryngeal cancer before and after operation were measured by radioimmunoassay. 20 cases of laryngeal benign lesions and 30 healthy adults as control. At the same time, the expression of EGF in the tissue was detected by APA immunohistochemistry. Results: The positive rate of EGF in serum of laryngeal cancer group was 73.7%. Preoperative serum EGF levels were significantly higher than those of benign laryngeal lesions and healthy controls (P <0.01). Postoperative serum EGF decreased to near normal. Two cases of postoperative recurrence with distant metastasis serum EGF again increased. High level of serum EGF and laryngeal cancer pathological grading and lymph node metastasis, and age, location of the lesion and clinical stage unrelated. Immunohistochemical EGF positive expression was 63.2%, and the pathological grade. Conclusion: Serum EGF can be used as a useful biomarker for the diagnosis, prognosis and follow-up of laryngeal cancer.