在植物育种程序和种质保存期间,常需要一种能鉴别不同品种的方法,这在育种后期尤其重要,因为此时有必要证明未来之新品种是否与现有品种不同。还有新品种推广时,种子必须高度一致,且遗传非常稳定。本实验室以往鉴别大麦品种主要是通过种子和植株性状的植物学分析,不过最近已在增加采用蛋白质及同工酶的电泳分离鉴别品种,由此开拓了大麦主要贮藏蛋白的多形性,可用于品种鉴别和醇溶蛋白位点的遗传分析。尽管贮藏蛋白确有变异,但用SDS—聚丙烯酰胺凝胶电泳(PAGE)区分特定的大麦品种一直不大成功,这或许是欧洲的许多大麦品种仅来源于少 Often, during plant breeding programs and germplasm preservation, a method of identifying different varieties is required, which is especially important later in the breeding season, as it is then necessary to demonstrate whether future new varieties will differ from the existing ones. There are new varieties to promote, the seeds must be highly consistent, and the genetic is very stable. In the past, the identification of barley cultivars in our laboratory was mainly based on the botanical analysis of the seed and plant traits. However, the use of protein and isoenzyme electrophoresis to separate and identify cultivars has led to the development of the polymorphism of the main storage proteins in barley. Genetic Identification of Varieties and Gliadin Loci. Although there is indeed a variation in storage proteins, the identification of specific barley varieties by SDS-polyacrylamide gel electrophoresis (PAGE) has been less successful, perhaps because many barley varieties in Europe are derived from less