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目的:探讨无翅型MMTV整合位点家族成员10a(Wnt10a)在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)和桥本合并甲状腺乳头癌(Hashimoto’s thyroiditis associated with papillary thyroid carcinoma)中的表达及其临床意义。方法:采用q RT-PCR方法检测Wnt10a m RNA在41例新鲜甲状腺组织(PTC组织18例,HT合并PTC组织12例,结节性甲状腺肿组织11例)中的表达;用免疫组化SP法检测Wnt10a蛋白在50例甲状腺石蜡标本(PTC组织20例,HT合并PTC组织20例,结节性甲状腺肿组织10例)中的表达。结果:1 RT-PCR Wnt10a m RNA在甲状腺癌组织中的表达明显高于其在良性甲状腺肿瘤组织中的表达(P<0.01),差异有显著性;Wnt10a m RNA在PTC、HT合并PTC组织中的阳性表达强度分别为2.49×10-5(0.68×10-5,15.28×10-5),1.26×10-5(0.97×10-5,13.73×10-5);P<0.05,差异具有显著性。Wnt10a m RNA在PTC、HT合并PTC组织中的表达分别是结节性甲状腺肿组织的9.98、2.69倍。2SP法:Wnt10a在甲状腺癌组织中的表达明显高于其在良性甲状腺肿瘤组织中的表达。Wnt10a m RNA和蛋白在甲状腺癌组织中的表达与肿瘤大小、临床分期、淋巴结转移等临床因素无明显关系(P>0.05),只与甲状腺癌组织的病理分型相关,(P<0.05)。结论:Wnt10a在甲状腺乳头状癌和桥本合并甲状腺乳头癌中呈现高表达,且有差异性,有望作为甲状腺癌的病理分型的参考指标,有助于甲状腺癌的早期诊断及病理分型的判定,使患者得到及时有效治疗。
OBJECTIVE: To investigate the expression of Wnt10a, a family member of wingless MMTV integration site in papillary thyroid carcinoma (PTC) and Hashimoto’s thyroiditis associated with papillary thyroid carcinoma significance. METHODS: The expression of Wnt10a m RNA in 41 cases of fresh thyroid tissue (18 cases of PTC, 12 cases of HT complicated with PTC and 11 cases of nodular goiter) was detected by q RT-PCR. The expression of Wnt10 mRNA was detected by immunohistochemical SP method The expression of Wnt10a protein was detected in 50 cases of thyroid paraffin specimens (20 cases of PTC, 20 cases of HT complicated with PTC and 10 cases of nodular goiter). Results: 1 RT-PCR Wnt10a m RNA expression in thyroid cancer was significantly higher than that in benign thyroid tumors (P <0.01), the difference was significant; Wnt10a m RNA in PTC, HT combined with PTC tissue The positive expression intensity was 2.49 × 10-5 (0.68 × 10-5,15.28 × 10-5), 1.26 × 10-5 (0.97 × 10-5,13.73 × 10-5), P <0.05, the difference was Significance. The expression of Wnt10a m RNA in PTC and HT with PTC was 9.98 and 2.69 times that of nodular goiter respectively. 2SP method: Wnt10a expression in thyroid cancer was significantly higher than its expression in benign thyroid tumors. The expression of Wnt10a m RNA and protein in thyroid cancer tissues was not related to the clinical factors such as tumor size, clinical stage and lymph node metastasis (P> 0.05), but correlated with the pathological type of thyroid cancer (P <0.05). CONCLUSION: Wnt10a is highly expressed in papillary thyroid carcinoma and Hashimoto’s thyroid papillary carcinoma, and it is expected to serve as a reference index for the pathological classification of thyroid carcinoma, which is helpful for the early diagnosis and pathological classification of thyroid carcinoma Judgment, so that patients receive timely and effective treatment.