论文部分内容阅读
电化学检测器的专一性可排除来自被氧化的代谢产物的干扰,检测限度是1～2 ng/ml,对MDL 72567(1～100ng)的线性回归方程的相关系数是0.9992,对硝苯啶(1～50ng)是0.997。浓度为50ng/ml时,当天或日间的变异系数分别为3.9(n=5)和6.0%(n=15)。本法可应用于测定狗的血药浓度。仪器:WISP710A自动进样器(Waters),Waters 590型泵,WatersμBondapak C18反相柱,LC-4电化学检测器(BAS)。条件:流动相0.07 M磷酸盐缓冲液(pH7.0):甲醇:乙腈(52∶36∶12),检测器的电位为1.00V,Ag/AgCl为参比电极,二氢吡啶还原为吡啶。MDL 72567和硝苯啶互为内标物。
The specificity of the electrochemical detector excludes the interference from oxidized metabolites with a limit of detection of 1 to 2 ng / ml and a linear regression of MDL 72567 (1 to 100 ng) with a correlation coefficient of 0.9992 for n-butanol The amount of pyridine (1 to 50 ng) is 0.997. At 50ng / ml, the coefficients of variation for the day or day were 3.9 (n = 5) and 6.0% (n = 15), respectively. This method can be applied to the determination of dog blood concentration. Instrument: WISP710A autosampler (Waters), Waters 590 pump, Watersμ Bondapak C18 reverse phase column, LC-4 electrochemical detector (BAS). Conditions: mobile phase 0.07 M phosphate buffer (pH7.0): methanol: acetonitrile (52:36:12), detector potential 1.00V, Ag / AgCl as the reference electrode, dihydropyridine reduced to pyridine. MDL 72567 and nifedipine are internal standards.