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目的:探讨前列腺素酶COX-2在宫颈癌及癌前组织中的表达及意义。方法:采用免疫组化SP法、半定量RT-PCR法及Western-blot法检测COX-2在35例宫颈上皮肉瘤样病变(CIN)、40例宫颈癌和5例正常宫颈组织中的表达情况。结果:①经免疫组化检测,COX-2蛋白在正常宫颈组织中不表达,在CIN宫颈组织阳性表达率分别为31.4%,在宫颈癌组织中阳性表达率分别为85.0%。三者之间差异具有显著性(P<0.05)。②经RT-PCR检测,正常宫颈组织中无COX-2 mRNA表达,CIN宫颈组织中COX-2 mRNA相对含量为0.291±0.214,宫颈癌组织中相对含量为0.357±0.173,三者之间差异具有显著性(P<0.05)。③经Western-blot检测,COX-2蛋白在宫颈癌Ⅰ、Ⅱ、Ⅲ期中相对含量分别为40.27±13.67,56.98±21.13和84.21±22.74,三者之间差异具有统计学意义(P<0.05)。结论:COX-2蛋白不表达于正常宫颈组织中。COX-2可能参与了宫颈癌早期的发生发展。宫颈组织中COX-2的表达可作为宫颈癌早期诊断及估计宫颈癌患者预后的一个指标。
Objective: To investigate the expression and significance of prostaglandin COX-2 in cervical cancer and precancerous tissues. Methods: The expressions of COX-2 in 35 cases of cervical epithelial sarcomatoid lesions (CIN), 40 cases of cervical cancer and 5 cases of normal cervical tissues were detected by immunohistochemical SP method, semi-quantitative RT-PCR and Western-blot. . RESULTS: ① The immunohistochemical staining showed that COX-2 protein was not expressed in normal cervical tissue, and the positive rate in cervical intraepithelial neoplasia was 31.4% and 85.0% respectively in cervical intraepithelial neoplasia. The difference between the three was significant (P <0.05). ② The expression of COX-2 mRNA in normal cervical tissue was not detected by RT-PCR, the relative content of COX-2 mRNA in cervical tissue of CIN was 0.291 ± 0.214, the relative content of cervical cancer was 0.357 ± 0.173, the difference between them was Significance (P <0.05). ③ The relative content of COX-2 protein in cervical cancer Ⅰ, Ⅱ, Ⅲ were 40.27 ± 13.67,56.98 ± 21.13 and 84.21 ± 22.74 respectively by Western-blot, the difference was statistically significant (P <0.05) . Conclusion: COX-2 protein is not expressed in normal cervical tissue. COX-2 may be involved in the early development of cervical cancer. The expression of COX-2 in cervical tissue can be used as an indicator of early diagnosis of cervical cancer and prognosis of cervical cancer.