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目的:研究骨骼肌细胞在不同时间的电刺激下产生的GLUT4基因与肌源性IL-6蛋白含量及其基因表达之间的关系。方法:以小鼠骨骼肌肌母细胞(C2C12)为研究对象,分为电刺激组和对照组。分别测定当刺激时间为45 min、60 min、75 min、90 min及120 min时C2C12肌管内糖原含量、GLUT4和IL-6 mRNA表达以及C2C12肌管上清液IL-6含量。结果:(1)电刺激各组GLUT4 mRNA表达均有所增加,其中60 min、75 min和120 min组显著高于对照组(P<0.05);电刺激75 min和120 min组IL-6 mRNA表达显著高于对照组(P<0.05);电刺激各组上清液IL-6含量均显著高于对照组(P<0.05)。(2)随电刺激时间增加,上述各项指标均先增加,后下降,90 min时达低谷。(3)随电刺激时间延长,C2C12肌管糖原含量逐渐减少,其中,刺激120分钟组糖原含量与对照组相比显著减少(P<0.05)。结论:(1)电刺激C2C12肌管对细胞糖代谢产生了一定影响;(2)电刺激C2C12肌管引起的GLUT4 mRNA表达的增加很可能与肌管收缩产生的IL-6有关。
OBJECTIVE: To study the relationship between GLUT4 gene and myogenic IL-6 protein content and gene expression in skeletal muscle cells under different electrical stimulation. Methods: Mouse skeletal muscle myoblasts (C2C12) were divided into electrical stimulation group and control group. The content of glycogen, the expression of GLUT4 and IL-6 mRNA in C2C12 myotubes and the content of IL-6 in C2C12 myotube supernatant were measured at stimulation time of 45 min, 60 min, 75 min, 90 min and 120 min respectively. Results: (1) The expression of GLUT4 mRNA in each group increased after 60 min, 75 min and 120 min stimulation (P <0.05), while the expression of IL-6 mRNA in 75 min and 120 min stimulation group (P <0.05). The content of IL-6 in the supernatant of each group was significantly higher than that of the control group (P <0.05). (2) With the increase of electrical stimulation time, the above indexes first increased, then decreased, reached the trough at 90 min. (3) The content of C2C12 myotubes gradually decreased with the prolongation of electrical stimulation, and the content of glycogen in 120 min stimulated group decreased significantly compared with the control group (P <0.05). Conclusion: (1) Electrical stimulation of C2C12 myotubes has a certain effect on the cellular glucose metabolism; (2) The increase of GLUT4 mRNA expression induced by C2C12 myotubes stimulation is likely to be related to IL-6 produced by myotube contraction.