论文部分内容阅读
目的研究不同条件的MEK抑制剂U0126对食管癌Eca109细胞生长的影响。方法 Western-blot检测不同浓度的抑制剂U0126对ERK1/2表达的影响;MTT检测抑制剂U0126在不同的作用条件下,对食管癌Eca109细胞生长的影响并筛选出最佳的作用条件;流式细胞术检测U0126作用后细胞的凋亡情况。结果 Western-blot检测不同浓度的U0126对ERK1/2表达抑制作用,提示最佳抑制浓度为50μmol。MTT检测结果为不含胎牛血清(FCS)的RPMI1640配制U0126,作用1小时对食管癌细胞的生长抑制最佳。流式细胞术检测显示细胞凋亡率为6.5%。结论不同作用条件下的抑制剂U0126,对食管癌细胞生长的影响不同。
Objective To investigate the effect of different conditions of MEK inhibitor U0126 on the growth of esophageal cancer Eca109 cells. Methods The effect of different concentrations of inhibitor U0126 on the expression of ERK1 / 2 was detected by Western-blot. The effect of inhibitor U0126 on the growth of esophageal carcinoma Eca109 cells was detected by MTT assay and the optimal conditions were selected. Cytometry was used to detect the apoptosis of U0126 cells. Results The inhibitory effect of different concentrations of U0126 on ERK1 / 2 expression was detected by Western-blot, suggesting that the optimal inhibitory concentration was 50μmol. The result of MTT assay showed that U0126 was formulated with RPMI1640 without Fetal Calf Serum (FCS), and the inhibition of growth for esophageal cancer cells for one hour was the best. Flow cytometry showed that the apoptosis rate was 6.5%. Conclusion U0126, an inhibitor of different effects, has different effects on the growth of esophageal cancer cells.