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目的探讨Bmi-1表达与食管鳞癌生物学行为之间的关系。方法应用免疫组化SP法和荧光定量PCR方法从蛋白和mRNA水平检测48例食管鳞癌组织中Bmi-1的表达。结果食管鳞癌组织中Bmi-1蛋白的表达量显著高于癌旁食管黏膜组织(P<0.01),mRNA水平表达与蛋白一致;Bmi-1蛋白高表达与组织分化程度及淋巴结转移有关(P<0.05),与年龄、性别、肿瘤大小无关(P>0.05)。结论 Bmi-1在食管鳞癌中高表达,与疾病进展密切相关,可作为预测食管癌生物学行为、判断预后的一个参考指标。
Objective To investigate the relationship between Bmi-1 expression and biological behavior of esophageal squamous cell carcinoma. Methods The expression of Bmi-1 in 48 cases of esophageal squamous cell carcinoma was detected by immunohistochemical SP method and fluorescent quantitative PCR method from protein and mRNA level. Results The expression of Bmi-1 protein in esophageal squamous cell carcinoma tissues was significantly higher than that in para-cancerous esophageal mucosa tissues (P <0.01). The expression of Bmi-1 protein was correlated with the degree of differentiation and lymph node metastasis <0.05), but not related to age, sex and tumor size (P> 0.05). Conclusion Bmi-1 is highly expressed in esophageal squamous cell carcinoma and is closely related to the progress of the disease. It may be used as a reference index to predict the biological behavior of esophageal cancer and prognosis.